Báo cáo Maintaining fruit quality and increasing shelf life of pomelo in the Mekong delta of Southern Vietnam

CARD PROJECT 050/04 VIE  
Improvement of export and domestic markets for  
Vietnamese fruit through improved post-harvest and  
supply chain management  
MAINTAINING FRUIT QUALITY AND INCREASING SHELF LIFE  
OF POMELO IN THE MEKONG DELTA OF SOUTHERN VIETNAM  
By Robert Nissen1, Nguyen Duy Duc2, Ms San Tram Anh2, Ms Tran Thi Kim Oanh2, Mr Vu Cong  
Khanh2 & Mr Ngo Van Binh2,  
1 Queensland Department of Primary Industries and Fisheries (DPI&F) , Maroochy Research  
Station, PO Box 5083 SCMC, Queensland, Australia, 4560.  
2 Southern Sub-Institute of Agricultural Engineering and Post-Harvest Technology (SIAEP), 54  
Tran Khanh Du Street, District 1, Ho Chi Minh City, Vietnam.  
1
MAINTAINING FRUIT QUALITY AND INCREASING SHELF LIFE  
OF POMELO IN THE MEKONG DELTA OF SOUTHERN VIETNAM  
Robert Nissen1, Nguyen Duy Duc2, Ms San Tram Anh2, Ms Tran Thi Kim Oanh2, Mr Vu Cong Khanh2 &  
Mr Ngo Van Binh2,  
1 Queensland Department of Primary Industries and Fisheries (DPI&F) , Maroochy Research Station,  
PO Box 5083 SCMC, Queensland, Australia, 4560.  
2 Southern Sub-Institute of Agricultural Engineering and Post-Harvest Technology (SIAEP), 54 Tran  
Khanh Du Street, District 1, Ho Chi Minh City, Vietnam.  
INTRODUCTION  
In Southern Vietnam, traditional wet market supply chains for agricultural fresh food are now giving way  
to new supermarket-led supply chain systems. The rapid transformation in the fruit and vegetables  
sector is due to the meteoric rise of supermarkets, hypermarkets, superstores, neighborhood stores,  
convenience stores, discount stores in Southern Vietnam. This change is impacting on both the  
upstream and downstream agricultural food supply chain participants through demands for safe, high  
quality and sustainable-produced fresh products and the greatest impact is being felt by the small  
farmers of southern Vietnam. Problems with traditional procurement supply chain practices include  
low- or no product standards, supply inconsistencies, highly variable transaction costs and limited or  
sequestered market information.  
Supermarkets are now setting new procurement practices and supply systems which focus on reducing  
costs and improving quality to enable them to sell at lower prices. This will allow them to win over  
consumers and obtain a larger share of their target market. The ability of many small farmers,  
collectors and wholesalers in the Mekong Delta of Vietnam to meet safe food levels and quality  
demands of domestic and overseas supermarkets can only be obtained through investing in  
improvements in their production and supply chain practices.  
Implementation of new production and post–harvest practices and the modernisation of these supply  
chains may prevent some small farmers from participating. Many small farmers will have to develop  
risk minimisation strategies, such as forming groups, implementing new crop management and  
production systems, improved packaging, more efficient transport methods and handling practices to  
provide a safe, competitively priced quality product. Understanding how to develop new supply chains  
and where to make changes is essential if farmers and all chain participants are to benefit.  
Many farmers are now realising that pomelo is a perishable fruit and traditional chains may have to  
change. Pomelo fruit suffer severely from moisture loss. Loading and unloading occurs at the local  
markets in the hot sun, speeding up the desiccating of the fruit. This moisture loss changes the fruit  
appearance, making the fruit less desirable, affecting its salability. High quality class fruit (extra or  
super class fruit and class 1 fruit) are often marketed with stalks attached. This is done to reassure  
customers that the fruit they are purchasing fresh produce. These stalks often break off during handling  
and transport and also draw moisture from the fruit. Farmers, collectors, traders, packaging agents and  
wholesalers estimates of fruit damage is from 1 to 2%, but surveys found that it is often above 10% due  
to moisture loss, handling damage and disease attack.  
2
Therefore, this CARD Project set up experiments to evaluate the benefits of developing new post-  
harvest practices to improved fruit quality by reducing moisture loss for the pomelo supply chains  
operating in southern Vietnam.  
METHODOLOGY  
Because pomelo fruit suffer from the loss of moisture, this CARD project set up an experiment to  
reduce this moisture loss and increase shelf life and retail value of pomelo fruit. An anti-transparent  
and vacuumed wrapping of fruit in polyethylene bags were trialed to determine if this moisture loss  
could be significantly reduced.  
The pomelo variety “Nam Roi” was chosen as the variety on which to conduct the experiments. Nam  
Roi is famous pomelo variety in Vietnam as the fruit are sought after by many Vietnamese consumers.  
It has special fruit qualities such as:-  
seedless  
yellow flesh colour  
the flesh is crisp and sweet  
TREATMENTS  
Fruit assessments were carried out at the SIAEP laboratory in Ho Chi Minh City. Treatments were  
applied to the pomelo fruit and fruit stored on the bench in the laboratory at SIAEP at about 25oC.  
Mature pomelo fruit purchased from the Thuy Duc Wholesale Markets in Ho Chi Minh City were used in  
this experiment. Experimental design consisted of three treatments of 10 fruit per rep sampled every 7  
days for 12 weeks. Treatments applied were:  
1. Control  
2. Citra Shine Special Wax applied to the fruit  
CITRASHINE is a shellac-based wax formulated with purified natural secretion and  
water-soluble emulsifying agents. It does not contain any solvents but water and all  
ingredients are approved for use on foodstuf by the Health regulations in most  
countries. This product is USFDA approved.  
CITRASHINE is stable and anti-transparent and the long-lasting shine which it  
provides improves citrus fruit appearance and skin resistance, increasing the fruit  
market value. In addition CITRASHINE controls dehydratation and prevents fruit from  
excessive weight loss. Fruits were cleaned by detergent deccosol before apply the  
wax to remove dirt, dust and foreign material.  
3. Vacuum sealed polyethylene plastic film applied to the fruit (wrapping)  
A Lavezzini Vacuum Packaging Systems, Model Boxer was used to wrap and seal the  
pomelo fruit in a 0.014 mm Liner Low Density Polyethylene (LLDPE) plastic film.  
FRUIT MEASUREMENTS  
Assessments undertaken were:  
Average fruit weight in grams and percentage moisture loss over time  
Skin colour change over time using a Minolta Chromometer CR 200 and expressed as L a b  
values  
Titrateable Acid measured using 10 grams of flesh in 100ml of double distilled water and  
macerated. This solution was then filtered through cotton wool and 10 mls of juice was  
collected and 2 drops of phenolphalayene indicator was added and titrated with Sodium  
Hydroxide (NaOH) 0.1 normal solution, until colour change occurred.  
3
Total soluble solids or degree Brix, was measured using a temperature compensation hand  
held Atago Refractometer Model N-1E.  
Vitamin C content was measured using Association of Analytical Chemists (AOAC)  
International method 967.21. Procedure used was:  
o Take 10 g of fruit flesh and grind with 5 ml Metaphosphoric-acetic solution.  
o Remove all sample and place into a 100ml flask and add Metaphosphoric-acetic  
solution until it reaches 100mls and shake  
o Filter through absorbent cotton or rapid paper  
o Take 10ml of the above solution and place into a 100ml glass beaker  
o Titrate this solution with 2,6 diclorophenol indophenolat natri until fast pink colour  
appears. This takes about 2 minutes to occur  
o Count amount of 2,6 DCPIP need to titrate 1mg acid ascorbic  
ƒ
+ Take 2 ml standard acid ascorbic solution add 5 ml  
Metaphosphoric-acetic  
ƒ
+Titrate with 2,6 diclorophenol indophenolat natri until fast pink  
colour appears in about 2 minutes (note number ml : y)  
o Titrate blank sample with 2,6 diclorophenol indophenolat natri until fast pink colour  
appears. This takes about 2 minutes to occur (note number ml = B)  
Calculation  
(X B).F.V.100  
v.P  
Content of Vitamin C =  
(mg/100gram sample)  
ƒ
ƒ
ƒ
ƒ
ƒ
X: ml 2,6 diclorophenol indophenolat natri titrate sample  
V: extracting solution volume ( V = 100ml)  
v: extracting solution volume to titrate (v = 7ml)  
P: amount of sample ( p = 10 gram)  
F: mg ascorbic acid equivalent 1ml standard 2,6 diclorophenol  
2
indophenolat natri ( F= ).  
Y
Taste evaluations were carried out by an expert panel of 12 people established at SIAEP  
laboratory using the hedonic scale (1-9):-  
1 = dislike very much  
3 = dislike  
5 = neither dislike or like  
7 = like  
9 = like very much  
RESULTS  
LOSS IN FRUIT WEIGHT (MOISTURE)  
Fruit weight loss was greater for the Control and Vacuum Wrapping treatments compared to the Citra  
Shine Special Wax treatment (Figure 1). Moisture loss for the Vacuum Wrapping and Citra Shine  
Special Wax treatments was reduced by 13% and 22% respectively compared to the control treatment.  
The control treatment and Vacuum Wrapping treatment fruit shelf life was 63 days and the fruit treated  
with Citra Shine Special Wax was 77 days.  
Control treated fruit at 63 days were extremely shriveled. This was due to sever moisture loss and  
disease had affected the internal eating quality of the fruit. The Vacuum Wrapped treated fruit were  
4
also shriveled, but the fruit had not desiccated to the same degree as the control treated fruit at day 63.  
The Citra Shine Special Wax treated fruit did not reach the same level of desiccation as the Vacuum  
Wrapped treated fruit until day 77. The Control treated fruit had the highest rate and the greatest  
moisture loss of the three treatments (Figure 2).  
Moisture loss followed a power curve (y=a+bxc) pattern for all treatments. See Figures 2, 3 and 4  
below.  
40  
Control  
Citra Shine Special  
Vacuum Wrapping  
30  
20  
10  
0
1
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Day  
Figure 1. Comparison of percentage moisture loss in Nam Roi Pomelo fruit between control, Citra  
Shine Special Wax and Vacuum Wrap treatments.  
5
40  
30  
20  
10  
0
Control  
(power) y=a+bxc  
r20.9989934473  
a=-3.15094878  
b=3.139496644  
c=0.602550012  
0
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Days  
Figure 2. Control treatment, fruit moisture loss over time  
40  
Citra Shine Special Wax  
30  
20  
10  
0
(power) y=a+bxc  
r2=0.99056254  
a=-2.33936990  
b=2.072603593  
c=0.672818004  
0
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Days  
Figure 3. Citra Shine Special Wax treatment, fruit moisture loss over time  
6
40  
30  
20  
10  
0
Vacuum Wrapping  
(power) y=a+bxc  
r2=0.9910450338  
a=-2.33936990  
b=2.072603593  
c=0.672818004  
0
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Days  
Figure 4. Vacuum wrapping treatment, fruit moisture loss over time  
End of shelf life is based on external appearance and the characteristics used are:  
fruit have a soft leathery feel  
fruit are highly shriveled due to moisture loss  
neck of the fruit is sunken and more pronounced  
skin colour changes from bright yellow colour to a dull deeper yellow  
disease area on infected fruit increase in size rapidly.  
Internal appearance indicators for end of shelf life is:-  
internal browning of the segments and albedo  
juice sacks are soft and spongy  
juice sacks are not easily separated from the segment  
disease areas are visible in the flesh.  
7
Figure 5. Control treated fruit day1.  
Figure 6. Citra Shine Special Wax treated fruit  
day 1.  
Figure 7. Vacuum Wrapped treated fruit day 1.  
8
Figure 8. Control treated fruit day 63.  
Figure 9. Citra Shine Special Wax treated fruit day 63.  
9
Figure 10. Vacuum wrapped treated fruit day 63.  
10  
COLOUR READINGS  
Minolta Chromameter CR 200 was used to determine skin colour changes of the sampled fruit. The  
colour space measurements are:- L* (positive values indicate lightness and negative values indicate  
darkness), a* (positive values indicate red as opposed to negative values indicate green), and b*  
(positive values indicate yellow and negative values indicate blue).  
Figure 11. L* a* b* colour space difference E* ab  
Lightness 25%  
Lightness 50%  
Lightness 75%  
Figure 12. Colour diagrammatic representation of L* a* b* colour space values  
Note: Colour show here may not be a true representation of colour observed by the naked eye as these colours patches are  
dependent computer, screen and printer settings used to display or print this report.  
SKIN COLOUR READINGS  
Traders, collectors, packing agents, wholesalers, retailers and consumers look at the skin colour, and  
use this as one of the determinates of fruit maturity and ripeness. Therefore, in this experiment we  
have tried to establish the effects of the anti-transparent and vacuum wrapping on skin colour.  
11  
Survey work conducted by the CARD Project 05004/ VIE found:  
Wholesalers prefer to purchase Nam Roi pomelo fruit that are light green in colour, as this they  
regard this as a sign of freshness and maturity. If fruit are too yellow they are regarded as  
being overripe. IF fruit are too green, they are  
Wholesalers also prefer to purchase class 1 pomelo fruit with stalks (>10cm) and leaves  
attached, this is a sign of freshness  
Under high temperature conditions in Vietnam pomelo fruit ripen quickly, lose moisture and  
shrivel, then breakdown due to disease (fruit rots)  
Table 2 provides a comparison of representative colour space over time taken of the Control, Citra  
Shine Special Wax and Vacuum Wrapping treatment fruit.  
Table 2. Skin colour readings from day 1 to day 77 for treatments applied for Nam Roi Pomelo.  
Day  
1
Control  
Citra Shine Special Wax  
L* 53.63; a* -16.17; b* 29.75  
L* 56.91; a* -15.43; b* 32.36  
L* 54.58; a* -15.40; b* 31.30  
L* 57.27; a* -16.12; b* 33.82  
L* 58.17; a* -15.15; b* 34.41  
Vacuum Wrapping  
L* 58.82; a* -12.41; b* 21.83  
L* 59.22; a* -12.36; b* 22.95  
L* 59.86; a* -12.03; b* 24.42  
L* 61.22; a* -12.42; b* 25.15  
L* 61.39 a* -12.35; b* 25.75  
Readings  
L* 51.67; a* -15.85; b* 29.57  
L* 52.18; a* -15.70; b* 31.43  
L* 54.09; a* -15.82; b* 32.48  
L* 56.31; a* -15.09; b* 35.40  
L* 58.77; a* -15.03; b* 38.20  
7
Readings  
14  
Readings  
21  
Readings  
28  
Readings  
12  
Table 2 (cont). Skin colour readings from day 1 to day 77 for treatments applied for Nam Roi Pomelo.  
Day  
35  
Control  
Citra Shine Special Wax  
L* 59.24; a* -15.06; b* 36.49  
L* 61.56; a* -14.04; b* 37.83  
L* 61.57; a* -13.06; b* 39.12  
L* 62.55; a* -10.97; b* 40.98  
L* 66.09; a* -8.27; b* 45.40  
L* 64.16; a* -6.13; b* 45.22  
L* 68.02; a* -3.18; b* 50.16  
Vacuum Wrapping  
L* 62.02; a* -11.36; b* 27.56  
L* 62.63; a* -10.66; b* 28.26  
L* 58.87; a* -8.89; b* 27.18  
L* 67.44; a*-7.65 ; b* 34.38  
L* 68.81; a* -5.67; b* 36.79  
Readings  
L* 59.11; a* -13.49; b* 39.99  
L* 62.35; a* -11.12; b* 43.65  
L* 63.79; a* -9.09; b* 47.27  
L*64.58; a* -6.80; b* 47.77  
L* 64.78; a* -4.32; b* 48.85  
42  
Readings  
49  
Readings  
56  
Readings  
63  
Readings  
70  
Readings  
77  
Readings  
The Vacuum Wrapped treated fruit reach a similar skin colour 7 days (1 week) after the Control Treated  
fruit (Table 2). Fruit treated with Citra Shine Special Wax reach a similar yellow skin colour 14 days (2  
weeks) later than the Control treated fruit (Table 2). Therefore, based on skin colour, the Citra Shine  
Special Wax increased the fruit shelf life by 2 weeks and the Vacuum Wrapping increased shelf life by 1  
week.  
All citrus are non-climacteric fruit, meaning that they ripen gradually over weeks or months. External  
color changes during ripening, but is a function of climate more than ripeness, and a very poor indicator  
of maturity. The best indices of maturity for citrus are internal Total Soluble Solids (oBrix or sugar),  
Titrateable acid content (acid), and the oBrix/acid ratio.  
13  
SKIN L* COLOUR SPACE  
We found the L* colour space readings for fruit that were Vacuum Wrapped in a liner of low-density  
polyethylene plastic film were very slightly altered were due to the reflective nature of the plastic if fruit  
were measured with the wrapping plastic on.  
Fruit from all treatments ripened and the skin colour changed from a darker colour (green) to lighter,  
brighter (Yellow) colour with no adverse affects. Fruit of the Vacuum Wrapping treatment did not  
change to a lighter colour at the same rate as the Control and Citra Shine Special Wax treated fruit  
(Figure 13). The Control, Citra Shine Special Wax and Vacuum Wrapped treatments were straight line  
linear relationships (y=a+bx). See Figures 14, 15 and 16. Initially, the change from a darker colour to a  
lighter colour was constant for all treatments from day 1 to day 14. The Vacuum Wrapping treatment  
rate of change slowed until day 49, then increased rapidly to a lighter colour from day 49 to day 63  
(Figure 13).  
70  
68  
66  
64  
62  
60  
58  
Control  
Citra Shine Special  
Vacuum Wrapping  
56  
54  
52  
50  
0
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Days  
Figure 13. Comparison of the average L* colour space values for the Control, Citra Shine Special Wax  
and Vacuum Wrapping Treatments for Nam Roi Pomelo.  
14  
70  
65  
60  
55  
50  
Control  
y=a+bx  
r2=0.9763023534  
a=51.45446086  
b=0.233276555  
0
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Days  
Figure 14. Control treatment L* colour space change over time  
70  
65  
60  
55  
50  
Citra Shine Special Wax  
y=a+bx  
2
r =0.9808400384  
a=52.613986  
b=0.19952952  
0
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Days  
Figure 15. Citra Shine Special Wax treatment L* colour space change over time  
15  
70  
65  
60  
55  
50  
Vacuum Wrapping  
y=a+bx  
r2=0.8837896690  
a=57.78614059  
b=0.148571500  
0
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Days  
Figure 15. Vacuum Wrapping treatment L* colour space change over time  
16  
SKIN A* COLOUR SPACE  
For the a* colour space (red to green), the Control and Vacuum Wrapped treated fruit remained green  
until day 28 when the fruit lost the green colour and change to a light yellow colour (Figure 16). All  
three treatments had similar power curve relationships. The Control treatment changed in colour was  
slow until day 28, and then increased rapidly until day 63 (Figure 17). The Citra Shine Special Wax  
treatment increased slowly until day 2, and then increased rapidly until day 77 (Figure 18). The  
Vacuum Wrapping was the slowest and slowly increased until day 35 and then increased rapidly until  
day 63 (Figure 19).  
-2  
Control  
Citra Shine Special Wax  
Vacuum Wrapping  
-4  
-6  
-8  
-10  
-12  
-14  
-16  
-18  
0
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Days  
Figure 16. Comparison of the average a* colour space values for the Control, Citra Shine Special Wax  
and Vacuum Wrapping Treatments for Nam Roi Pomelo.  
17  
0
-5  
Control  
(power)y=a+bxc  
r2=0.99255473  
a=-16.029398  
b=0.0005508  
c=2.4113227  
-10  
-15  
-20  
0
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Days  
Figure 17. Control treatment a* colour space change over time  
0
Citra Shine Special Wax  
(power)y=a+bxc  
r2=0.96121494  
a=-15.867733  
b=0.00011966  
c=2.66249  
-5  
-10  
-15  
-20  
0
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Days  
Figure 18. Citra Shine Special Wax treatment a* colour space change over time  
18  
0
-5  
Vacuum Wrapping  
(power)y=a+bxc  
r2=0.98863022  
a=-12.452764  
b=0. 673678e-06  
c=3.1207941  
-10  
-15  
-20  
0
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Days  
Figure 19. Vacuum Wrapping treatment a* colour space change over time  
19  
SKIN B* COLOUR SPACE  
For the b* colour space readings, all three treatments had a straight line linear relationship and  
changed colour from blue to yellow colour (Figure 20). The skin b* colour space for the Control treated  
fruit changed from green to full yellow in 49 days (Figure 21), the Vacuum Wrapping fruit changed from  
green to full yellow in 56 days (Figure 22) and the Citra Shine Special Wax treated fruit changed from  
green to full yellow in 63 days (Figure 23).  
55  
Control  
Citra Shine Special Wax  
Vacuum Wrapping  
50  
45  
40  
35  
30  
25  
0
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Days  
Figure 20. Comparison of the average b* colour space values for the Control, Citra Shine Special Wax  
and Vacuum Wrapping Treatments for Nam Roi Pomelo.  
20  
55  
50  
45  
40  
35  
30  
25  
Control  
y=a+bx  
r2=0.98358781  
a=28.74766  
b=0.330902975  
0
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Days  
Figure 21. Control treatment b* colour space change over time  
55  
50  
45  
40  
35  
30  
25  
Citra Shine Special Wax  
y=a+bx  
r2=0.95109987  
a=28.564892  
b=0.2463527  
0
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Days  
Figure 22. Citra Shine Special Wax treatment b* colour space change over time  
21  
55  
50  
45  
40  
35  
30  
25  
Vacuum Wrapping  
y=a+bx  
r2=0.91038995  
a=20.7378876  
b=0.218001025  
0
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Days  
Figure 23. Vacuum Wrapping treatment b* colour space change over time  
22  
O
TOTAL SOLUBLE SOLIDS (TSS) OR DEGREE BRIX ( BRIX)  
For this experiment, no relationships could be established between time and change in Total Soluble  
o
Solids (oBrix). Figure 24 shows the Control treatment reaching a peak of 13 Brix on day 49 then  
o
dropping sharply to 9 Brix on days 56 and 63. This drop was due to the fruit being over ripe and  
breaking down internally. The Citra Shine Special Wax and Vacuum Wrapping treatments oBrix levels  
continued to rise from day 1 reaching 12 oBrix, even when the fruit had reach the end of their shelf life  
on day 63 and day 77 respectively (Figure 24).  
14  
Control  
Citra Shine Special Wax  
Vacumm Wrapping  
13  
12  
11  
10  
9
8
0
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Days  
Figure 24. Comparison of the average Total Soluble Solids (oBrix) for the Control, Citra Shine Special  
Wax and Vacuum Wrapping Treatments for Nam Roi Pomelo.  
TITRATEABLE ACID CONTENT OF FLESH (TA, %)  
For this experiment no relationships between time and change in titrateable acid content of the flesh  
was established. For all treatments fruit acid initially increased then dropped sharply. The decrease in  
acid content is consistent with the literature on post-harvest ripening process of citrus fruit. The acid is  
used in the respiration process for preserving the citrus fruit.  
The Control treatment fruit increased in acidity by 16%, peaking on day 21. Fruit then slowly decreased  
by 3% on day 35. The acidity then dropped sharply by 11% on day 42 before dropping sharply again  
by 20% on day 63 (Figure 25).  
Fruit treated with Vacuum Wrapping also increased in acid by 16% on day 14, peaking at 0.86% acid.  
Fruit then slowly decreased by 6% on day 21, stabilised for 3 weeks before dropping sharply by 25% to  
reach a low on day 63.  
The Citra Shine Special Wax treated fruit had a significantly lower acidity. The initial rate of increase  
was only half that of the Control and Vacuum Wrapping treatments. The acidity level for this treatment  
23  
peaked at 0.79%, about 9% lower that the Control and Vacuum Wrapping treatments. The acid level  
decreased at a slower rate compared to the Control and Vacuum Wrapping treatments. Fruit acid then  
decreased by 25% to be 0.60% on day 77. The amount of decrease is similar to the decrease in acidity  
for the Control and Vacuum Wrapping treatments, 20% and 25% respectively.  
0.90  
0.85  
0.80  
0.75  
0.70  
0.65  
Day vs Control  
0.60  
0.55  
Day vs Citra Shine Special Wax  
Day vs Vacuum Wrapping  
0
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Days  
Figure 25. Comparison of the average Titrateable Acid Content of Flesh (TA, %) for the Control, Citra  
Shine Special Wax and Vacuum Wrapping Treatments for Nam Roi Pomelo.  
VITAMIN C CONTENT (MG/100G)  
Vitamin C (ascorbic acid), is one of the most important vitamins found in citrus juices. Temperature and  
storage time affects Vitamin C content of the pomelo juice. Fructose one of the major sugars found in  
orange juice, can also cause Vitamin C breakdown. The higher the fructose content the greater the  
loss of Vitamin C in the fruit. Conversely, higher acid levels of citric and malic acids will stabilize  
Vitamin C levels.  
For this experiment, power curve relationships between time and change in Vitamin C content of the  
flesh was established for the Control and Citra Shine Special Wax Treatments (Figure 23 and 24). For  
the Vacuum Wrapping treatment, no relationships could be established.  
24  
58  
56  
54  
52  
50  
48  
46  
Control  
Citra Shine Special Wax  
Vaccum Wrapping  
0
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Days  
Figure 15. Comparison of the average Vitamin C content (mg/100g) between treatments for Nam Roi  
Pomelo.  
For the Control treatment the Vitamin C content starting at 50.79mg/100g and by day 63, increased  
11% to 56.61mg/100g. The Citra Shine Special Wax treatment dropped slightly, from 50.79 mg/100g  
on day 1 to 49.43mg/100g on days 35 to 42, before increasing 12% to 57.15mg/100g on day 77. The  
Vacuum Wrapping treatment remained at about 50.5 mg/100g for the firs 3 weeks (days 7, 14 and 21)  
before rising to a peak on day 35 of 52.9mg/100g then falling 15% to a low of about 45mg/100g on  
days 56 and 63.  
25  
60  
58  
56  
54  
52  
50  
48  
46  
Control  
(power) y=a+bxc  
r2=0.99144725  
a=50.515258  
b=0.0021529695  
c=1.9328596  
0
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Days  
Figure 23. Control treatment change in Vitamin C content over time  
60  
58  
56  
54  
52  
50  
48  
46  
Citra Shine Special Wax  
(power) y=a+bxc  
r2=0.90240036  
a=49.982626  
b=0.370021e-07  
c=3.8613208  
0
20  
40  
60  
80  
Days  
Figure 24. Citra Shine Special Wax treatment change in Vitamin C content over time  
26  
60  
58  
56  
54  
52  
50  
48  
46  
0
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Days  
Figure 25. Vacuum Wrapping treatment change in Vitamin C content over time  
TASTE EVALUATIONS  
For this experiment power curve relationships between time and change in taste of the flesh was  
established for the Control and Vacuum Wrapping Treatments (Figures 26, 27 and 29). For the Citra  
Shine Special Wax treatment no relationships could be established (Figure 28). For the Control and  
Vacuum Wrapping treatments the taste of the fruit decreased with each passing week from rating 5.5 at  
day 1 to rating 1 at day 63. The Citra Shine Special Wax treatment taste remained constant for the first  
3 weeks at rating 5.5 then increased strongly over 4 weeks where it remained until day 77 (Figures 26  
and 28).  
The flesh was still editable for all treatments on day 63 (Figures 30 to 35).  
27  
8
7
6
5
4
3
2
1
0
Control  
Citra Shine Special Wax  
Vacuum Wrapping  
0
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Days  
Figure 26. Comparison of the average taste evaluations for the Control, Citra Shine Special Wax and  
Vacuum Wrapping Treatments for Nam Roi Pomelo.  
Control  
8
6
4
2
0
y=a+bx3  
r2=0.95187934  
a=5.2677875  
b=-1.917299e-05  
0
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Days  
Figure 27. Control treatment change in taste ratings over time  
28  
8
6
4
2
0
0
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Days  
Figure 28. Citra Shine Special Wax treatment change in taste ratings over time  
8
Vacuum Wrapping  
y=a+bx3  
r2=0.8752014484  
a=4.9926205  
6
b=1.2851301e-05  
4
2
0
0
7
14  
21  
28  
35  
42  
49  
56  
63  
70  
77  
Days  
Figure 29. Vacuum Wrapping treatment change in taste ratings over time  
29  
Figure 30. Control treatment fruit day 63  
Figure 31. Citra Shine Special Wax treatment fruit day 63  
30  

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